Sr. Research Specialist II (Biophysics)

The Beckman Center for Cryo-EM is seeking a Sr. Research Specialist II who is an expert electron microscopist to support the center’s operations in cryo-electron tomography (cryo-ET), focusing on lamella preparation and cryo-correlation with focused-ion beam scanning electron microscopy (FIB-SEM) and cryo-correlative light electron microscopy (Cryo-CLEM). The Beckman Center houses state-of-the-art instrumentation and support infrastructure including the Titan Krios equipped with a Falcon 4i direct electron detector and Selectris energy filter, a Glacios equipped with a Falcon 4i direct electron detector, and an Aquilos II cryo-CLEM/FIB-SEM with iFLM. The Sr. Research Specialist II uses specialized scientific knowledge and experience to make decisions, trains/guides other laboratory personnel, and provides simple to complex troubleshooting. Specific Duties & Responsibilities * Training and assisting users with grid preparation, FIB-SEM, and cryo-CLEM for 3D cryo-ET analysis (primarily utilizing the Aquilos II and associated equipment). * Day-to-day instrument operation, including FIB milling of cells and tissue, image acquisition, and microscope maintenance. * Providing user support for image processing and tomographic analysis. * Maintain high-throughput software pipelines. * Develop, design and manage specialized projects and/or instrumentation, and coordinates own objectives. * Utilize specialized knowledge to set up and run sophisticated instrumentation and interpret results. * Act as consultant to refine or adapt methodologies to fit research objectives. * Collaborate in development of new protocols and uses of equipment. * Advise investigators on experimental design and research methods. * Co-author/author publications. * Contribute original thoughts, hypotheses, and analysis to write reports, abstracts and manuscripts. * Provide significant input to research design and data collection and interpretation based on advanced knowledge/ understanding of activity. * Independently perform research assays specific to the research laboratory interests. * Collaborate with other lab personnel, managers, and faculty investigators. * Independently develop protocol and criteria. * Make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction. * Consider resources and laboratory priorities when making decision. * Other duties as assigned. Additional Skills, Knowledge and Abilities * Significant hands-on FIB-SEM experience, experience in cryo-electron microscopy (cryo-ET / cryo-EM), image processing, and structure modeling or comparable experience and expertise with the relevant techniques. * The successful candidate must have strong interpersonal communication skills and be able to effectively work with faculty, staff, postdoctoral fellows, and students from a variety of scientific and cultural backgrounds. Technical Skills & Expected Level of Proficiency * Data Management and Analysis - Developing * Experimental Design and Execution - Developing * Laboratory Troubleshooting and Technical Problem Solving - Developing * Project Management - Developing * Protocol Development and Optimization - Developing * Scientific Interpretation and Consultation - Developing * Scientific Writing - Developing The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs. Minimum Qualifications * Bachelor's Degree in Biology, Chemistry or related field. * Five years of experience in a comparable laboratory performing relevant techniques. * Additional education may substitute for required experience and additional related experience may substitute for required education permitted by the JHU equivalency formula beyond a high school diploma/graduation equivalent, to the extent Preferred Qualifications * Ph.D. Degree in a relevant...